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KMID : 0381120090310050361
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Genes and Genomics
2009 Volume.31 No. 5 p.361 ~ p.368
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Gene Mapping and Expression Analysis of a Novel Mutant reproduce organs absent (roa) in Rice
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Wang Nan
Sang Xian-Chun
Li Yun-Feng
Ling Ying-Hua
Zhao Fang-Ming
Yang Zheng-Lin
He Guang-Hua
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Abstract
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Mutant plays an important role in function analysis in plant. A rice flower mutant reproduce organs absent (roa), showing a stable inheritance during several years of study, was identified in rice (Oryza sativa L. ssp. Indica) cultivar Jinhui10 treated with EMS and used in this study. This mutant showed following: elongated palea and pedicle; absence of inner three whorls of floral organs; multi-whorls glume like organs inside the lemma/palea; spikelet meristem like organ upon the pedicle. These phenotypes suggested that ROA is a key gene in rice spikelet development. Genetic analysis confirmed that the mutant traits were controlled by a single recessive nuclear gene. By gene mapping, ROA was restricted between two SSR markers RM221 and RM1342 on the chromosome 2. It concluded that ROA was a novel gene involving in flower development in rice. Besides, the mutation of ROA influenced the transcription level of floral homeotic genes; the expression of floral homeotic genes decreased in roa panicle compared with wild-type, and it suggested that ROA affected flower development by influencing the expression of floral homeotic genes.
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KEYWORD
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rice (Oryza sativa L.), flower mutant, gene mapping, ABCE model
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